Figure 3. Tissue bacterial burden in the lungs and spleen.

The copies of B. pseudomallei gene/g of tissue was determined for the spleen and lungs from each animal at the time of death or when euthanatized 28 days after exposure to B. pseudomallei. Copies/g were determined by real-time PCR and each symbol represents the results for an individual tissue. Triangle represent doxycycline treated mice and squares represent WFI treated controls. Open symbols represent spleen tissue results and filled symbols represent lung tissue results. The bars represent the mean copies/g in a given tissue for a given treatment group. Doxycycline treatment significantly reduced the copies/g in both the spleen and lungs (p < 0.0001).