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. 2013 Jan 28;3:1142. doi: 10.1038/srep01142

Figure 6. Effects of 4-PBA on the activations of NF-κB/IκBα p65 and on the HIF-1α protein levels in lung tissues of LPS-treated mice.

Figure 6

Sampling was performed at 48 hours after LPS treatment in 0.9% NaCl solution (saline)-treated mice administered with drug vehicle (PBS) (SAL+PBS), LPS-treated mice administered with PBS (LPS+PBS), or LPS-treated mice administered with 4-PBA (LPS+PBA). Representative Western blots of NF-κB p65 (a) and IκBα (c) in the lung tissues. (b) Analyses of band intensity on films are presented as the relative ratio of the level of NF-κB p65 in each group to the level in SAL+PBS. (d) In case of IκBα, the relative ratio was presented to actin. The relative ratio of each protein in the lung tissues of SAL+PBS is arbitrarily presented as 1. (e) Representative Western blots of HIF-1α and HIF-1β in nuclear protein extracts from the lung tissues. (f) Analyses of band intensity on films are presented as the relative ratio of HIF-1α to HIF-1β in LPS+PBS or LPS+PBA to those in SAL+PBS. The relative ratio of each protein in the lung tissues of SAL+PBS is arbitrarily presented as 1. Bars represent mean ± SEM from 8 mice per group. #P < 0.05 versus SAL+PBS; *P < 0.05 versus LPS+PBS.