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. 2012 Sep 6;120(11):1585–1591. doi: 10.1289/ehp.1205316

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TDCPP and gDNA methylation during cleavage. gDNA extracted from TDCPP-treated embryos (+)—but not vehicle-treated embryos (–)—at the end of cleavage (2 hpf) was completely digested by methylation-sensitive restriction endonucleases (white arrows), suggesting that normal gDNA methylation at 2 hpf was absent in TDCPP-treated embryos. Gels are representative of three independent gDNA samples per time point and treatment.