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. 2012 Jun 22;51(31):7680–7683. doi: 10.1002/anie.201202660

Figure 1.

Figure 1

An assay for the hydrophobic pocket binding. a) The hydrophobic pocket on the surface of PBD participates in the binding of the PBIP1 peptide. The hydrogen bond between the phenol of Y417 and D72PBIP1 is essential for pocket binding. b) The principle of the use of different protein forms to distinguish the binding modes in a high-throughput fluorescence-based thermal shift (FTS) assay.