Table 1.
The results of the assay for the series of modified peptides (R-DPPLHSpTA-NH2). ΔTm values are shown for the wild-type (WT) and double-mutant Y417A/Y421A (DM) protein; the difference between them (ΔΔTm) and the KD value against the wild-type protein is also shown. The color code corresponds to that used in Figure 2 a,b
| R | ΔTm [K] | KD [nm] | |||
|---|---|---|---|---|---|
| WT | DM | ΔΔTm | |||
| 1 a |  |
12.9 | 9.7 | 3.2 | 160 |
| 1 b |  |
9.8 | 9.7 | 0.1 | 960 |
| 2 a |  |
10.3 | 10.0 | 0.3 | 1100 |
| 2 b |  |
10.9 | 10.7 | 0.2 | 1200 |
| 2 c |  |
11.5 | 10.5 | 1.0 | 1100 |
| 2 d |  |
11.0 | 10.5 | 0.5 | 950 |
| 2 e |  |
13.6 | 10.3 | 3.3 | 160 |
| 2 f |  |
9.3 | 8.8 | 0.5 | 1200 |
| 3 a |  |
12.5 | 11.5 | 1.0 | 240 |
| 3 b |  |
11.6 | 11.3 | 0.3 | 310 |
| 3 c |  |
12.2 | 11.0 | 1.2 | 190 |
| 3 d |  |
15.4 | 11.4 | 4.0 | 66 |
| 3 e |  |
11.6 | 11.0 | 0.6 | 280 |
| 3 f |  |
15.8 | 11.2 | 4.8 | 21 |
| 3 g |  |
9.9 | 9.7 | 0.2 | 790 |
| 3 h |  |
12.7 | 10.2 | 2.5 | 140 |
| 3 i |  |
9.9 | 8.8 | 1.1 | 1000 |
| 3 j |  |
16.0 | 11.9 | 4.1 | 20 |
| 3 k |  |
16.0 | 11.2 | 4.8 | 40 |