Table 1.
Plant species list used for determining sensitivity of the general plant primers c A49325 (Taberlet et al. 1991) and trnL110R (Borsch et al. 2003). The lowest template numbers (copies per reaction) where a PCR product could be obtained are given as detection limits
Detection limits of plant DNA | ||||
---|---|---|---|---|
Plant species | Plant family | Functional group | Plant DNA only | Plus wireworm DNA |
Achillea millefolium* | Asteraceae | Herb | 100 | 100 |
Fagopyrum esculentum | Polygonaceae | Herb | 100 | 100 |
Lolium perenne* | Poaceae | Grass | 100 | 100 |
Lupinus angustifolius | Fabaceae | Legume | 100 | 100 |
Phaseolus coccineus | Fabaceae | Legume | 200 | 200 |
Pimpinella major* | Apiaceae | Herb | 100 | 100 |
Plantago lanceolata* | Plantaginaceae | Herb | 100 | 100 |
Sinapis alba | Brassicaceae | Herb | 100 | 100 |
Taraxacum officinale* | Asteraceae | Herb | 100 | 100 |
Trifolium pratense* | Fabaceae | Legume | 100 | 100 |
Trifolium repens | Fabaceae | Legume | 100 | 100 |
Triticum aestivum | Poaceae | Grass | 100 | 100 |
Zea mays | Poaceae | Grass | 100 | 100 |
Plant species that were fed to wireworms in the feeding experiment with living grassland plants.