Table 1.

Development of Spectral Flow Cytometry

Reference	Approach	Comments
Wade et al, 1979(Wade et al., 1979)	Grating spectrograph and silicon intensified detector	Measured average spectra of many cells
Steen and Stokke, 1986(Steen and Stokke, 1986)	Grating monochromometer and PMT	Measured average intensity at 10 nm intervals
Buican, 1990(Buican, 1990)		Fourier transform FC
Gauci et al, 1996(Gauci et al., 1996)	Prism and intensified photodiode array	Measured spectra of single cells and microspheres
Fuller and Sweedler, 1996(Fuller and Sweedler, 1996)	Grating spectrograph and CCD	Measured spectra of single microspheres an liposomes
Asbury et al, 1996(Asbury et al., 1996)	Scanning monochromometer and PMT	Constructed population average spectra from many single cells, single wavelength measurements
Dubelaar et al, 1999(Dubelaar et al., 1999)	Grating spectrograph and a 7 pixel hybrid PMT	Used 3 of 7 detector elements to measure light scatter and two colors of fluorescence
Isailovic et al, 2005(Isailovic et al., 2005)	Grating and ICCD camera	Measured spectra of bacterial cells in a capillary flow system
Robinson et al, 2005(Robinson et al., 2005);Gregori et al 2012(Grégori et al., 2011)	Prism or grating and multianode PMT	Measured spectra of fluorescence-stained cells, PCA analysis
Goddard et al, 2006(Goddard et al., 2006)	Grating and CCD	Measured spectra from single beads and cells
Watson et al, 2008(Watson et al., 2008); Nolan et al, 2012(Nolan and Sebba, 2011)	Imaging spectrograph and CCD	Measured fluorescence and SERS spectra from individual beads and cells, PCA and linear unmixing analysis