Fig. 2.

Raw data and variables measured during conditioning experiments. Top panel shows typical experiments for 5–40 min PIC followed 60 min later by a second OGD event (black bar, 20 min). The vertical dotted line shows the start of the first OGD event (i.e. the PIC event). The caudate brain slice is superfused with oxygenated aCSF for 45 min and then subjected to 0–40 min OGD. After about 5 min we see a massive and fast increase in dopamine of approximately 15 μM (C–G). Panel A shows 125 min of data, with subsequent panels showing slightly longer traces, depending on the length of PIC, up to 165 min for panel G. The lower panel shows the dopamine efflux event in more detail and the parameters we measure; T-on is the time from the start of OGD until dopamine efflux; T-peak is the time from the start of dopamine efflux until peak dopamine efflux; peak-DA is the maximum dopamine concentration measured; δDA/δt is the rate of change of dopamine efflux (nM/s).