Fig. 1.

Crry deletion reduces CFH and tau phosphorylation. (a) Immunoblot of hippocampal protein lysates probed for CFH, left hand panel. Densitometry was performed and is shown in bar graph, right. Specificity of the CFH antibody is shown on wild type and CFH knockout brain tissue, inset panel. The apparent molecular weight of the CFH immunoreactive band is between ∼165 kDa. (b) Immunoblots showing MC6 immunoreactivity, top panel, and total tau immunoreactivity using DAKO A0024, bottom panel. Densitometry values obtained for MC6 were normalised to total tau protein values and are presented in the bar graph, right. Significance values were determined by Student's T-test. n = 10 per group. Error bars = SEM.