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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: J Proteome Res. 2012 Nov 27;11(12):5843–5855. doi: 10.1021/pr3006167

Figure 2.

Figure 2

Distribution of proteins and lipid raft marker proteins across the Optiprep gradient fractions analyzed by immunoblotting. Lipid raft were isolated from whole body BBMV as described in Materials and Methods. An equal volume of each gradient fraction was analyzed by 10% SDS-PAGE followed by silver staining (A), and immunoblotting with anti-Flotillin-1 (B), anti-AgAPN1, (C) and anti- Cry4Ba (D) antisera.