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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: J Proteome Res. 2012 Nov 27;11(12):5843–5855. doi: 10.1021/pr3006167

Figure 3.

Figure 3

Effect of MBCD on Protein, Cholesterol, Cry4Ba toxin, APN and ALP distribution in Aedes lipid raft fractions. Isolation of lipid rafts from A. aegypti BBMV with pre incubation of MBCD followed by detergent solubilization. Triton X-100 insoluble complexes were prepared (see Materials and Methods) centrifuged through a 5%:30%:40% Optiprep step gradient and 1-ml fractions assayed for Cholesterol and total protein content (A), APN & ALP enzyme activity (B), Flot-1 immunoblot (C) and Cry4Ba toxin blot (D). Treatment of BBMV with 20 mM MBCD had no effect on the cholesterol and protein content of lipid raft fractions.