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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: J Proteome Res. 2012 Nov 27;11(12):5843–5855. doi: 10.1021/pr3006167

Figure 4.

Figure 4

Schematic representation of Aedes lipid rafts isolation and mass spectrometry analysis procedures. Lipid rafts were prepared from the BBMV as described in Materials and Methods. A representative centrifuge tube picture of the gradient with opalescent lipid rafts band obtained after centrifugation is shown (A). Detergent insoluble proteins (15 μg) were resolved on 10% SDS-PAGE and stained with Deep Purple™ total protein stain. Gel bands were sliced equally and digested with trypsin. The resulting peptides were analyzed by LC-MS/MS (B).