Fig. 2.

mRNA levels of Tcbr in the whole body and the midgut of T. castaneum determined by quantitative reverse-transcriptase real-time PCR (qRT-PCR). Samples were collected at 12 h interval during the final instar larval and pupal stages. Total RNA was extracted from pools of three larvae for each treatment. The Y-axis denotes expression levels normalized using the rp49 levels as an internal control. Mean ± SE of two independent experiments with three replications each are shown.