Fig. 3.

mRNA levels of Tcbr in dsRNA injected and control insects. dsRNA injections were done during penultimate larval stage (a), final instar larval stage [24 h (b) and 72 h AEFL (c)]. The insects were sampled at 4–5 days after injection. See text for details. Total RNA was extracted from individual three larvae for each treatment. cDNAs prepared from the RNA were used in qRT-PCR. The relative expression levels of Tcbr mRNA were determined using the levels of rp49 as an internal control. Mean ± SE of three independent experiments are shown.