Fig. 6.

Knock-down in Tcbr expression results in expression of larval, pupal and adult characters in the same insects. Tcbr dsRNA was injected into the final instar larvae (at 24 h) and SEM images of various organs are compared with wild-type larval/pupal/adult counterparts (a–l). (a–d) Head and thoracic region, (e–h) antennae and (i–l) mouthparts. All panels show ventral side of insects orienting head to the top and abdomen to the bottom. (a–d) well developed thoracic sternal plates (white star). (e–h) Flagellar segments of the antenna (white arrow) and hygroscopic receptors wherever present (white star). (i–l) Mandibles (Mn), labrum (Lb), maxilla (Mx) and labium (Lm); absence of gaps between incisors observed in the pupal mandibles (j, white arrow line); interlocking of mandibles (k and l, white star). Scale bar: (a–d) 100 μm; (e–h) 20 μm; (i–l) 100 μm.