Fig. 9.

Knock-down in Tcbr expression affects midgut remodeling. Tcbr dsRNA or malE (control) dsRNA was injected at 24 and 72 h AEFL. Cross-sections (CS, 10 μm thick) of midguts dissected from insects dissected 5 days after dsRNA injection and nuclear stained with DAPI are shown (a–d). (a and b) The comparison of CS of midgut dissected from insects injected with Tcbr dsRNA at 24 h AEFL. (c and d) Midguts dissected from insects injected with Tcbr dsRNA at 72 h AEFL. The pupal/adult cells (pink arrow head) and larval cells (white arrow) are shown (a–d). Note the formation of crypt-like evagination (d, yellow star) in the midgut dissected from larvae injected with Tcbr dsRNA at 72 h AEFL. Scale bar: 50 μm.