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. Author manuscript; available in PMC: 2013 Jan 28.
Published in final edited form as: Int J Dev Biol. 2010;54(4):755–760. doi: 10.1387/ijdb.082668ss

Fig. 2. Localisation of SOX2 transcripts and SOX2 protein in testicular carcinoma in situ (CIS).

Fig. 2

(A) In situ hybridisation showing the localisation of SOX2 transcripts in CIS testis (arrows), and weak staining in Sertoli cells (arrowheads). Top right corner shows sense control. (B) immunostaining with the AF2018 goat SOX2 antibody from R&D Systems, showing lack of the protein in CIS cells (arrows) but its presence in Sertoli cell nuclei (arrowheads), (C) immunostaining with the AB5603 SOX2 antibody from Millipore showing Sertoli cell nuclear staining (arrowheads) and variable speckled nuclear staining of CIS cells (arrows), and (D) OCT4 used as a marker of CIS cells (arrows) in the serial section for C. Size bars represent 20 μm.