TABLE 1.
SOX2 EXPRESSION BY IMMUNOHISTOCHEMISTRY (IHC) AND IN SITU HYBRIDISATION (ISH) DURING NORMAL HUMAN TESTICULAR DEVELOPMENT AND TGCTS
| IHC with AB5603 |
IHC with AF2018 |
Transcripts by ISH |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tissue and cell type | Age | N | Staining score |
Intensity and location | N | Staining score |
Intensity and location | N | Staining score |
Intensity and location |
| Fetal and prepubertal testis | ||||||||||
| Gonocytes/prespermatogonia | 15-41 wg | 4 | − | Negative (weak cytoplasmic background staining) |
3 | − | None | ND | ||
| Infantile/prepubertal spermatogonia | 2 m - 10 y | 7 | − | Negative (weak cytoplasmic background staining) |
6 | − | None | ND | ||
| Adult testis (including normal tubules in vicinity of CIS) | ||||||||||
| Spermatogonia | Adult | 9 | − to + | Medium nuclear (in a subset of cells) |
9 | − | None | 3 | +− | Weak cytoplasmic |
| Sertoli, undifferentiated in CIS tubules | Adult | 9 | ++ | Medium nuclear | 9 | ++ | Strong nuclear | 2 | ++ | Moderate cytoplasmic |
| Spermatocytes | Adult | 9 | +/− | Weak nuclear | 9 | − | None | 3 | ++ | Weak cytoplasmic and nuclear (some signal in sense controls) |
| Spermatids | Adult | 9 | − | None | 9 | − | None | 3 | + | Weak cytoplasmic |
| Testicular neoplasms | ||||||||||
| CIS | Adult | 11 | ++ | Weak nuclear | 11 | − | None | 2 | ++ | Heterogeneous cytoplasmic |
| Seminoma | Adult | 6 | − | None | 6 | − | None | 3 | ++ | Moderate cytoplasmic |
| Embryonal carcinoma | Adult | 5 | +++ | Strong nuclear; | 5 | +++ | Strong nuclear | 3 | +++ | Strong cytoplasmic |
| Teratoma | Adult | 4 | ++ | Strong nuclear (in a subset of cells positive for OCT4) |
4 | ++ | Strong nuclear (in a subset of cells positive for OCT4) |
ND | ||
| Testicular B cell lymphoma | 58 y | 1 | − | None | 1 | − | None | ND | ||
The proportion of positively stained cells was assessed using a semi-quantitative score (as described in Methods). All fetal, infantile and prepubertal sections were from formalin fixed archival tissues not suitable for ISH. Abbreviations: CIS, carcinoma in situ ; m, months; N, number; ND, not done; wg, weeks of gestation; y, years.