Table 2.

Particle size, polydispersity index, and zeta potential of different liposomes prepared by conventional and SCF-CO₂ methods

Liposome	Mean diameter (nm)	Polydispersity index	Zeta potential (mV)
Non-filtered
SCF-S100	1137.96 ± 131.39	0.34 ± 0.02	−7.73 ± 3.38
SCF-E80	747.04 ± 82.30	0.30 ± 0.03	−30.77 ± 7.00
Film-S100	2155.90 ± 373.55	0.61 ± 0.09	−7.92 ± 5.55
Film-E80	1543.01 ± 66.31	0.51 ± 0.10	−22.4 ± 9.33
Filtered
SCF-S100	188.93 ± 17.00	0.22 ± 0.02	−6.82 ± 2.96
SCF-E80	166.58 ± 9.60	0.18 ± 0.01	−30.72 ± 1.92
Film-S100	244.30 ± 44.15	0.22 ± 0.02	−5.76 ± 0.56
Film-E80	198.87 ± 26.24	0.20 ± 0.03	−19.25 ± 6.12

Notes: “Filtered” represents liposomes passed through a 1.0 μm pore size PTFE syringe filter before analysis. Values denote the mean ± standard deviation of six separate sets of experiments.

Abbreviations: SCF-CO₂, supercritical fluid of carbon dioxide; SCF-S100, supercritical fluid liposomes prepared using Lipoid S100; SCF-E80, supercritical fluid liposomes prepared using Lipoid E80; Film-S100, conventional liposomes prepared using Lipoid S100; Film-E80, conventional liposomes prepared using Lipoid E80; PTFE, polytetrafluoroethylene.