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. 2013 Jan;15(1):23–38. doi: 10.1593/neo.121502

Figure 3.

Figure 3

Adiponectin inhibits key nodes of leptin signaling in breast cancer cells. (A) Breast cancer cells were pretreated with 10 µg/ml adiponectin (Adn) for 24 hours followed by 100 ng/ml leptin (L) treatment for various intervals of time. (B) Breast cancer cells were pretreated with 100 ng/ml leptin (L) followed by 10 µg/ml adiponectin (Adn) treatment for various intervals of time. Total protein was isolated and equal amounts of proteins were resolved by SDS-PAGE and subjected to immunoblot analysis using antibodies for pERK and pAkt. The membranes were reblotted using total ERK and Akt antibody. Anti-actin antibody was used as a control. The blots are representative of multiple independent experiments. Adiponectin treatment decreased leptin-induced phosphorylation of ERK and Akt in breast cancer cells. (C, D) Breast cancer cells were pretreated with 10 µg/ml adiponectin (Adn) for 24 hours followed by 100 ng/ml leptin (Lpn) treatment for various intervals of time and subjected to ERK and Akt activity assay. Adiponectin pretreatment decreased leptin-induced Akt and ERK activity.