Figure 4.

The proportion of TILs contacting TuDCs is enhanced after chemotherapy. OTI-DsRed T cells were adoptively transferred into CD11c-YFP transgenic mice bearing MCA-OVA tumor treated or not with CP. Seven days after adoptive transfer, tumors were carefully orientated and sectioned to define the periphery and the center of the tumor. (A) Representative tumor sections are shown and the distance from the periphery is indicated. OTI T cells are red, YFP⁺ cells are green, and tumor parenchyma is stained by DAPI (blue). (B) Representative examples of the OTI T cell/YFP⁺ distance measurement (white bars) between the center of the T cell and its proximal YFP⁺ cell. Graph represents the frequency distribution of the distances (right). Mean radius of OTI T cell is 5 µm, and conjugates with a distance less than 5 µm are considered as contacting cells [n = 304 OTI cells for Ctrl (white bars) and n = 880 OTI cells for CP-treated mice (black bars) pooled from two to three different tumors].