Figure 2.

miR156 Promotes Bud and Leafy Gametophore Formation.

(A) Schematic of the MIM156 overexpression construct. pro, promoter; NosT and CaMVT, Nos and cauliflower mosaic virus terminator, respectively; At4, At4 gene from Arabidopsis; 108, 108 targeting locus from the P. patens genome (Schaefer and Zrÿd, 1997).

(B) to (F) Delay of bud and gametophore production in MIM156 lines. Four-week-old plants (four independent MIM156 lines [B] to [E] and the wild type (WT; [F])] are shown. Bars = 3 mm.

(G) to (H) Increased accumulation of miR156 targets in MIM156 plants at the time of maximal bud initiation. RNAs from spore-germinated 3-week-old tissues (G) and 5-week-old tissues (H) were analyzed by qRT-PCR as in Figure 1. Expression relative to the wild type was plotted. Error bars indicate sd from three biological replicates. Significant differences from the wild type were analyzed by t test (*P ≤ 0.05 and **P ≤ 0.01).