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. 2012 Dec 21;24(12):4837–4849. doi: 10.1105/tpc.112.103176

Figure 5.

Figure 5.

Overexpression of MIR390C Represses Bud and Leafy Gametophore Formation.

(A) Expression pattern of miR390, tasiARF, and tasiAP2 small RNAs in wild-type plants at the indicated ages, measured by stem loop qRT-PCR. The relative accumulation levels to the 1-week sample were plotted. Error bars indicate sd from three biological replicates. Analyses of significant differences are shown in Supplemental Figure 10A online.

(B) Expression pattern of the target mRNAs of tasiARF and tasiAP2 in the samples as in (A), measured by qRT-PCR. The relative accumulation levels to the 1-week sample were plotted. Error bars indicate sd from three biological replicates. a, 1s14_392V6.1; b, 1s280_72V6.1; c, 1s6_75V6.1; d, 1s5_432V6.1; e, 1s74_86V6.1. Analyses of significant differences are shown in Supplemental Figure 10B online.

(C) Overexpression of MIR390C. RNAs from protonemata tissues of the wild type (WT) and MIR390c OE were analyzed by stem loop–mediated qRT-PCR. Error bars indicate sd from three biological replicates. Significant differences from wild type were analyzed by t test (*P ≤ 0.05 and **P ≤ 0.01).

(D) Rates of bud and gametophore appearance. Seven-day-old protonemal tissues of the wild type and MIR390C OE were inoculated on media. The numbers of buds and subsequent gametophores in 16 colonies were counted every 2 d. Error bars represent the se values for those 16 colonies. Significant differences from wild type on each day were analyzed by t test (*P ≤ 0.05). The raw data for day 16 is in Supplemental Table 2 online.

(E) Stem loop qRT-PCR of tasiARF, tasiAP2, and tasiZNF in 3-week-old plants. Error bars indicate sd from three biological replicates. Significant differences from the wild type were analyzed by t test (*P ≤ 0.05 and **P ≤ 0.01).

(F) Accumulation of tasiARF and tasiAP2 target mRNAs in 3-week-old plants measured by qRT-PCR. Error bars indicate sd from three biological replicates. a to e are as in (B). Significant differences from the wild type were analyzed by t test (*P ≤ 0.05 and **P ≤ 0.01).

(G) Accumulation of the tasiZNF target mRNA 1s286_43V6.1, measured by qRT-PCR. Error bars indicate sd from three biological replicates. No significant differences relative to the wild type were found (t test, P > 0.05).