Figure 4.

Gene Structure Comparison and Phylogenetic Analysis between Bn-CLG1A Homologs Identified in B. napus, B. oleracea, B. rapa, and Arabidopsis.

Gene structure comparison (A) and phylogenetic analysis (B). Deduced amino acid sequences of these homologs were aligned with ClustalX (Thompson et al., 1997). The phylogeny analysis was performed with MEGA version 4.1 using the neighbor-joining method (Tamura et al., 2007). The percentage of replicates trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The legend of gene structure in (A) is the same as in Figure 3. The green arrow indicates tandem duplication of the Rzfp2C gene copy into Rzfp2’C in the C genome of B. napus (Bn-Rzfp2’C) and B. oleracea (Bo-Rzfp2’C). Rzfp, RING zinc finger protein.