Figure 2.

CHX-Sensitive IAA Biosynthesis Is Rapidly Induced after Addition of 5% Glc.

(A) Incorporation of deuterium label into the IAA pool after treatment of 10 DAG seedlings with medium containing 30% deuterated water and 0% (circles) or 5% Glc (triangles). The seedlings were pretreated for 4 h in liquid medium. Increased IAA synthesis rates were observed up to 20 h after the beginning of treatment. Inset: Short incubation with 60% deuterated water. The asterisk denotes statistical difference at 95% confidence level.

(B) Inhibition of protein synthesis with CHX abolishes Glc induction of IAA synthesis. Gray bars indicate IAA biosynthesis rate without sugar treatment and dark bars after incubation in medium containing 5% Glc. Error bars denote se (n = 4).