Figure 3.

Transcript Analysis Shows That Members of the Auxin Biosynthesis Gene Families CYP79B and YUCCA Are Induced by Exogenous Glc.

(A) Gene expression analysis of a subset of genes directly related to IAA biosynthesis using the ATH1 array. Whole seedlings were pretreated for 4 h in liquid medium and then treated for 15 h in medium containing 0, 1, or 5% Glc. Both pretreated (0 h) and treated samples are included in the analysis. Bars indicate fold change in logarithmic values compared with a 15-h treatment with no sugar.

(B) Quantitative PCR expression analysis for selected Glc-inducible genes using gene-specific primers. The transcript levels are expressed against the average expression of four reference genes. Treatment as in (A) with 15-h treatment in 5% mannitol added. Error bars indicate se (n = 3 or 4; in 20-h mannitol treatment, n = 1).