Figure 3.

IBH1 Interacts with HBI1 in Vitro and in Vivo.

(A) Yeast two-hybrid assays of interactions between indicated IBH1 proteins and PRE1, BEE2, or HBI1. The AS domain is required for the IBH1 interaction with HBI1 but not for interaction with PRE1.

(B) A gel blot of MBP and MBP-IBH1 was probed with GST-HBI1 followed by horseradish peroxidase–labeled anti-GST antibody or stained with Ponceau S (Stain).

(C) Coimmunoprecipitation assays show IBH1 interacts with HBI1 in plant cells. 35S:HBI1-YFP was transformed into the protoplasts prepared from the 35S:IBH1-myc transgenic plants. Immunoprecipitation was performed using anti-YFP antibody, and immunoblots were probed with anti-myc or anti-YFP antibodies.

[See online article for color version of this figure.]