PREs, IBH1, and HBI1 Mediate Hypocotyl Elongation Responses to BR, GA, Temperature, and Light.
(A) and (B) Wild-type (WT) and transgenic 35S:pre-amiR, 35S:IBH1-myc, and 35S:HBI1-SRDX plants were grown under constant light for 7 d in half-strength MS medium with (+) or without (−) indicated hormones: 100 nM BR
(A), 1 μM GA3, and 100 nM PAC
(B). Right panels: The hypocotyl lengths were measured from at least 15 plants. Error bars represent sd.
(C) Wild-type and transgenic 35S:pre-amiR, 35S:IBH1-myc, and 35S:HBI1-SRDX plants were grown at 20 or 29°C for 7 d. The hypocotyl lengths were measured from at least 15 plants. Error bars represent sd.
(D) Wild-type and transgenic 35S:pre-amiR, 35S:IBH1-myc, and 35S:HBI1-SRDX plants were grown under different light conditions for 7 d. The hypocotyl lengths and relative lengths (percentage of length in the dark) were calculated from at least 10 plants. Error bars represent sd.