Table 1. Female Gametophyte Phenotypes of Heterozygous mab1 (RNAi) Silencing Lines.
| Genotype | na | One (Chalazal) Nucleus | Two Attached Nuclei | Two Adjacent Nuclei | Σ Mutant FG1-FG2 Phenotypes | Collapsed FGs | Fully Developed FGs (FG7) |
|---|---|---|---|---|---|---|---|
| RNAi | 80 (100%) | 8 (10%) | 6 (7.5%) | 14 (17.5%) | 35% | 5 (6.3%) | 47 (58.7%) |
| (line 1149) | |||||||
| RNAi | 55 (100%) | 2 (3.6%) | 2 (3.6%) | 4 (7.2%) | 14.4% | 12 (21.8%) | 35 (63.8%) |
| (line 143) | |||||||
| Wild-type | 63 (100%) | 0 | 0 | 0 | 0% | 3 (4.8%) | 60 (95.2%) |
| (A188xH99) |
Ovules were manually sectioned and examined by confocal laser scanning microscopy after period acid-Schiff staining. Two independent mab1 (RNAi) and a wild-type line of the same genetic background were compared. Note that ovules were randomly selected and studied at a silk length of 3 to 8 mm when wild-type ovaries contained fully differentiated mature female gametophytes. FG, female gametophyte.
a
Total no. of dissected and scanned ovules; only ovule sections generating a complete three-dimensional female gametophyte stack were counted.