Table 2. Immunohistological analysis of male meiosis in MAB1-RNAi lines.
| Meiosis I |
Meiosis II |
∑ Meiosis | ||||
| Genotype | na | Normal | Abnormalb | Normal | Abnormalc | defects |
| RNAi (line 143) | 44 (100%) | 18 (40.9%) | 8 (18.2%) | 5 (11.4%) | 13 (29.5%) | 47.7% |
| RNAi (line 1149) | 60 (100%) | 20 (33.3%) | 10 (16.7%) | 14 (23.3%) | 16 (26.7%) | 43.4% |
| Wild-type (A188) | 65 (100%) | 34 (52.3%) | 1 (1.5%) | 29 (44.7%) | 1 (1.5%) | 3% |
DAPI-stained microsporocytes with fluorescently labeled microtubules were counted from three wild-type and three RNAi plants.
a
Total No. of microspores counted.
b
Abnormalities at meiosis I refer to chromosome segregation defects and occurance of micronuclei after cytokinesis.
c
Abnormalities at meiosis II refer to shorter and misoriented spindles leading to abnormal tetrads or in the case of wt only to abnormal tetrads.