Figure 6.

VICTR Associates with Nuclear Pool of EDS1 and PAD4.

Confocal analysis of N. benthamiana leaves agroinfiltrated with BiFC vector combinations of VICTR with EDS1 or PAD4. These vector combinations use the N-terminal fragment of an enhanced version of YFP (nVenus) and the C-terminal fragment of CFP (cCFP), which when reconstituted gives yellow fluorescence (Hu and Kerppola, 2003). Localization of indicated molecular complexes is shown by yellow fluorescence. Red fluorescence indicates free RFP. Panels show images with separate YFP, RFP, and an overlap of YFP + RFP fluorescence. Arrowheads indicate the nucleus. The experiment was repeated with identical results (n = 3). Bars = 20 μm.