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. 2013 Jan 7;110(4):1500–1505. doi: 10.1073/pnas.1217121110

Fig. 5.

Fig. 5.

ABHD12 is a major brain LPS lipase. (A) Membrane homogenates from HEK293T cells transfected with a murine ABHD12 cDNA (gray bars) displayed increased hydrolytic activity compared with mock-transfected cell membranes (black bars) toward several lysophospholipids and other singly acylated glycerolipids (e.g., MAG, BMP). In contrast, doubly acylated glycerolipids (e.g., DAG, PA, PC, PE, PI, PG, PS) were not hydrolyzed by ABHD12. See Table S7 for more information on lipid species tested as ABHD12 substrates. (Inset) Active ABHD12 expression in transfected cells was confirmed by ABPP with the FP-rhodamine probe. (B) ABHD12−/− brain membrane homogenates (black bars) displayed significantly reduced hydrolytic activity toward 18:1 LPS, LPG, LPI, and BMP compared with ABHD12+/+ brain homogenates (white bars). Data are presented as average values ± SEM (n = 3 per group). *P < 0.05; **P < 0.01; ***P < 0.001 vs. mock or ABHD12+/+ homogenates (Student’s t test).