Fig. 2.

Pharmacological dissection of intra- and extracellular CA activity in oocytes injected with CA IV–cRNA. Cytosolic H⁺ (H⁺_i) recording (A) and the rate of H⁺_i change (B), and membrane surface H⁺ (H⁺_s) recording (C) and amplitude of H⁺_s (D) during addition and removal of 5% CO₂/25 mM HCO₃⁻, respectively, without inhibitor and in the presence of 0.5–1 µM BZA or 30 µM EZA. The rate of H⁺_i change (E) and the amplitude of H⁺_s (F) were differently affected by prior injection of 1 µM BZA into the oocyte but equally sensitive to application of 30 µM EZA.