Fig. 2.

Transport activities of Bv-PPase. (A) Na⁺ accumulated in IMV in 1 min after PP_i addition. (B) H⁺ transport, determined using ACMA fluorescence. The following additions were made where indicated: 100 μM AMDP, 5 μM CCCP, 2 μM valinomycin, 20 μM ETH157. (C) Comparison of Na⁺ transport activities of Bv-PPase and A. caccae Na⁺-PPase. Hydrolytic activities of Bv-PPase and A. caccae Na⁺-PPase were 15 and 16 nmol·min⁻¹·mg⁻¹, respectively. (D) Comparison of H⁺ transport activities of Bv-PPase and L. biflexa H⁺-PPase. Hydrolytic activities of Bv-PPase and L. biflexa H⁺-PPase under these conditions were 34 and 31 nmol·min⁻¹·mg⁻¹, respectively. The error bars in A and C are the SDs of three measurements.