Fig. 4.

Phylogeny of membrane PPase protein sequences. (A) Experimentally characterized Na⁺-PPases are labeled with plain numbers, Na⁺,H⁺-PPases with squares, and H⁺-PPases with asterisks. The dotted line highlights the K⁺-independent subfamily, whereas letters A, B, C, and D indicate the nodes of different clades within the K⁺-dependent subfamily. The taxon numbers 6 and 7 represent M. mazei Na⁺-PPase and Bv-PPase, respectively. Other taxon names are provided in Table S2. (B) Phylogeny of the Na⁺,H⁺-PPase subfamily. The tree was rooted to M. mazei Na⁺-PPase. The enzymes characterized in this study are indicated in bold. Nodes are supplemented with clade credibility values. (Scale bars: 0.2 substitutions per residue.)