Table 1.
Kinetic parameters for Bv-PPase activation by Na+ and K+
Activator | pH | Ka, mM | V1, nmol·min−1·mg−1 | V2, nmol·min−1·mg−1 | Ki, mM |
Na+ | 7.2 | 0.57 ± 0.06/33 ± 3 | <3/<3 | 420 ± 20/360 ± 20 | 340 ± 60/320 ± 30 |
Na+ | 5.5 | 2.0 ± 0.3/21 ± 2 | <2/<2 | 160 ± 10/56 ± 5 | 310 ± 70/n.d. |
K+ | 7.2 | 11 ± 3 | 150 ± 10 | 390 ± 20 | |
K+ | 5.5 | 12 ± 3 | 44 ± 4 | 170 ± 10 |
Parameter values separated by the slashed line were measured in the presence and absence of 50 mM K+, respectively. K+ binding experiments were conducted in the presence of 50 mM Na+. Parameter values are reported with SD. n.d., not determined.