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. 2013 Jan 7;110(4):1404–1409. doi: 10.1073/pnas.1206761110

Fig. 1.

Fig. 1.

CU1276 is a Dicer-dependent tRNA fragment expressed in mature B cells. (A) Cloning frequency of CU1276 from naïve (N), GC, and memory (M) B cells purified from human tonsil, and from the Burkitt’s lymphoma cell line Ramos (RA). Data are derived from analysis of previously published small RNA libraries (6). (B) FASTA sequences of annotated human tRNAs with perfect match to the CU1276 small RNA. CU1276 sequence is highlighted in bold. (C) Northern blot analysis of total RNA from GC B cells, Ramos, and 293T cells transiently transfected with empty vector or a vector encoding for the Gly(GCC) chr1.tRNA68. The three primary bands correspond to the 22-nt tRNA fragment CU1276, the 74-nt mature tRNA, and a high molecular weight tRNA primary transcript. See also Fig. S1. (D) Western blot and Northern blot analyses of 293T cells stably expressing control shRNA (shCTRL) or a pool of three Dicer-targeting shRNA (shDicer), transiently transfected with empty or Gly(GCC) chr1.tRNA68 vector. Ethidium bromide (EtBr) staining and immunoblotting for ACTB were used as loading controls for Northern blot and Western blot, respectively.