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. 2013 Jan 7;110(4):1345–1350. doi: 10.1073/pnas.1213738110

Fig. 4.

Fig. 4.

UBXD8 binds ATGL and impairs ATGL-dependent LD turnover. (A) UBXD8 suppressed LD degradation induced by ATGL overexpression. Total LD area per cell was quantified in HeLa cells expressing the indicated constructs. (B) ATGL is required for LD up-regulation by UBXD8. LD area per cell was quantified in WT or ATGL−/− MEFs expressing a control plasmid or UBXD8-S. (C) UBXD8 interacts with ATGL. Immunoblot analysis of UBXD8 complexes immunopurified from detergent-solubilized LD-enriched fractions isolated from oleate-treated HEK293 cells. (D) Assessment of the ATGL–UBXD8 interaction by bimolecular fluorescence complementation. HEK293 cells transiently transfected with the indicated C-terminal (Yc) or N-terminal (Yn) “split YFP” constructs were treated with 200 µM oleate for 24 h and analyzed by flow cytometry.