Table 3.
List of primers used
| Gene | Primer | Sequence 5’→3’ | Fragment size (bp) | |
|---|---|---|---|---|
| |
oligo(−dT) anchor primer |
|
GACCACGCGTATCGATGTCGAC(T)16V |
|
|
FNS II |
FNS1 |
F |
CCCAACACCATGAATACACTCC |
|
| |
FNS4 |
R |
TCACCACTGAGAGTTCTCTCATGG |
|
|
FNS II |
FNSIIRub-ex |
F |
CCCAACACCATGAATACA C |
|
| |
|
R |
GAAGCGAAGGTAAACACA C |
|
|
FNS II |
Dah.FNSII.lang |
F |
CAACACCATGAATACACTCCTAGTACTCC |
|
| |
|
R |
CTTAGACTGAAGTAAACATCGAATGTGGAGAG |
|
|
FNS II |
qPCR |
F |
GCATCCAGAATCTCGGCCATT |
|
| |
qPCR |
R |
TCGTTGTCACTTCTCGCACTAG |
|
|
Actin |
qPCR |
F |
GGAAAAGATTTGGCATCACACTTTC |
103 |
| |
qPCR |
R |
AGCCTTAGGATTTAAAGGTGCCTCA |
|
|
GAPDH |
qPCR |
F |
AGGCTGTTGGTAAGGTGTTGC |
141 |
| |
qPCR |
R |
GCAGCCTTGATCTGCTCATAAG |
|
|
CHS1 |
qPCR |
F |
GCGCGTATATGGCACCTTCG |
198 |
| |
qPCR |
R |
GGCCGAGGAGCTTGGTGAGT |
|
|
CHS2 |
qPCR |
F |
ACGTTTCGTGGACCGACGGAT |
185 |
| |
qPCR |
R |
CAGCCCCACCTCCCTCAAG |
|
|
CHI |
qPCR |
F |
ACTTCCCTCCCGGCTCCTCT |
182 |
| |
qPCR |
R |
TTGTTTGGCTGCTGGGGAAACAC |
|
|
FHT |
qPCR |
F |
CCACAGCCCGACCTGACACT |
202 |
| |
qPCR |
R |
CGTTCTTGAACCGCCCATTG |
|
|
DFR |
qPCR |
F |
GCCAAAGCCAAGACGGTCAA |
192 |
| |
qPCR |
R |
CGTTGCCTTCCATGCTGCTT |
|
|
ANS |
qPCR |
F |
GCTGCAGGCGAGGGTTTCTT |
119 |
| |
qPCR |
R |
GTCGACGGCCAAATGGTGAG |
|
|
F3’H |
qPCR |
F |
AACTGCCTTGCTATTGTACGTACTG |
147 |
| |
qPCR |
R |
TTTTCCGCCAGGGCTGCC |
|
| MaT |
qPCR |
F |
TTGGAATATGCCGACCGATG |
112 |
| |
qPCR |
R |
CCTTTCCCCACCCAAAATCA |
|
|
Delila |
qPCR |
F |
ATCTCACAAACGCCGAGTGGTATT |
158 |
| |
qPCR |
R |
TGCTAGAAGAGAGCGGCAGAAGA |
|
|
IVS |
qPCR |
F |
CGTCGTCGAGAGAAGCTAAACGA |
151 |
| |
qPCR |
R |
GACATCGTGCTTCCAAATCCTGAA |
|
|
MYB1 |
qPCR |
F |
TTGAGGAAAGGTTCATGGTCAGC |
145 |
| |
qPCR |
R |
AATTTGTCCATCGTAGCCTGCAAC |
|
|
MYB2 |
qPCR |
F |
ACCCTCCTCCAACCACTCATCAT |
160 |
| |
qPCR |
R |
TGAGGTGGGCTCATTCTCAACTC |
|
|
R3MYB |
qPCR |
F |
ATGGCCGTGAGAAGGAAATTGATG |
153 |
| |
qPCR |
R |
CAAGGAGGTCCAACATGACAAC |
|
|
WDR1 |
qPCR |
F |
CGGTTGCGGAACTAGAGAGAC |
155 |
| |
qPCR |
R |
TGACATCGGGTCAATCCCATTAGG |
|
|
WDR2 |
qPCR |
F |
CAGAGGCATCAGTCGAGTGTGA |
149 |
| qPCR | R | GCCAATATCGGATCCAATCCACCT |
Abbrev.: F: forward, R: reverse.