Table 1.
Assays | PERC | EARC | AERC | 95ERC | WRC | GSH |
---|---|---|---|---|---|---|
Anti-lipid peroxidation |
13.99 ± 2.11e |
4.55 ± 0.44 b |
14.92 ± 4.31 d |
10.62 ± 0.97 c |
19.18 ± 2.04 f |
0.028 ± 0.00 a * |
DNA protective effect |
- |
1905.51 ± 177.54 c |
14397.18 ± 1075.01 d |
1090.11 ± 21.25 b |
- |
114.42 ± 2.66 a ** |
·OH |
561.19 ± 21.16 f |
111.55 ± 1.32 c |
132.32 ± 0.15 d |
84.30 ± 1.02 b |
233.45 ± 15.70 e |
37.67 ± 0.67 a ** |
·O2- |
322.81 ± 14.90 b |
306.68 ± 0.87 b |
284.36 ± 11.43 b |
301.08 ± 8.34 b |
650.65 ± 59.55 c |
81.65 ± 5.21 a |
Chelating |
191.86 ± 1.84 b |
319.20 ± 6.36 c |
872.71 ± 30.63 e |
553.34 ± 12.59 d |
2289.11 ± 100.00 f |
107.86 ± 0.31 a *** |
DPPH· |
813.00 ± 225.17 d |
227.09 ± 10.14 b |
260.34 ± 11.37 c |
254.73 ± 4.33 c |
229.17 ± 5.81 b |
5.13 ± 1.19 a ** |
ABTS·+ |
395.65 ± 77.34 e |
72.10 ± 1.32 b |
96.39 ± 1.12 d |
94.38 ± 2.45 d |
83.58 ± 0.81 c |
4.76 ± 0.28 a |
Fe3+ reducing | 533.12 ± 54.25 e | 159.35 ± 1.37 b | 317.06 ± 3.47 c | 156.19 ± 2.12 b | 488.75 ± 18.6 0 d | 51.89 ± 1.29 a |
IC50 value is defined as the concentration of 50% effect percentage and calculated by linear regression analysis and expressed as mean ± SD (n = 3). The linear regression was analyzed by Origin 6.0 professional software. Means values with different superscripts in the same row are significantly different (p < 0.05), while with same superscripts are not significantly different (p < 0.05). *The positive control was BHA, instead of GSH. ** The positive control was Trolox, instead of GSH. *** The positive control was Sodium citrate. Results were analyzed by independent samples. PERC, petroleum ether extract of rhizoma Cimicifugae; EARC, ethyl acetate extract of rhizoma Cimicifugae; AERC, absolute ethanol extract of rhizoma Cimicifugae; 95ERC, 95% ethanol extract of rhizoma Cimicifugae; WRC, water extract of rhizoma Cimicifugae. --: Cannot be detected.