Table 1.
Active and passive AIRs chosen for the docking experiments
|
Active and passive AIRs | ||
|---|---|---|
| Docking Run | active AIRs | passive AIRs |
| Baseline (BL) |
|
|
|
Rpn13 |
S55, L56, L73, I74, |
auto |
| |
F76, P77, D78, D79, |
|
| |
K99, A100, G101 |
|
|
ubiquitin |
K6, L8, I44, F45, |
auto |
| |
A46, G47, K48, Q49, |
|
| |
H68, V70, R72 |
|
| LP/MS filter (Ubiquitin CPORT) |
|
|
|
Rpn13 |
D41, K42, D53, D54, |
auto |
| |
E70, D79, E81, K99 |
|
|
ubiquitin |
- |
F4, K6-K11, P19, Q31, |
| |
|
E34-Q40, R42, I44-Q49, R54, |
| |
|
T55, S57-N60, Q62-T66, H68, |
| |
|
V70, L71-G76 |
| LP/MS (Ubiquitin CPORT) |
|
|
|
Rpn13 |
D53, D54, S55, D78, |
auto |
| |
D79, F98, K99 |
|
|
ubiquitin |
- |
F4, K6-K11, P19, Q31, |
| |
|
E34-Q40, R42, I44-Q49, R54, |
| |
|
T55, S57-N60, Q62-T66, H68, |
| |
|
V70, L71-G76 |
| chemical shift perturbations |
|
|
|
Rpn13 |
S55, F76, D78, K99, |
auto |
| |
G101, R104 |
|
|
ubiquitin |
L8, F45, A46, G47, |
auto |
| |
K48, Q49, H68, V70 |
|
| CPORT |
|
|
|
Rpn13 |
Y22-E25, R27, M31, |
auto |
| |
T36-P40, Q50-L56, H58, |
|
| |
V69, D71-I74, |
|
| |
F76, P77, D79, V85, C88-S90, |
|
| |
V93, V95, K103, L105, F106, |
|
| |
W108, E126, C127, N129, N130 |
|
|
ubiquitin |
F4, K6-K11, P19, Q31, |
auto |
| |
E34-Q40, R42, I44-Q49, R54, |
|
| |
T55, S57-N60, Q62-T66, H68, |
|
| V70, L71-G76 | ||