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. 2013 Jan 28;8(1):e54577. doi: 10.1371/journal.pone.0054577

Figure 5. Time course of Andro treatment on caspases and Bcl-2 family proteins expressed in DU145 cells.

Figure 5

The cells were incubated with 0–80 µM of Andro for 24 or 48 h, harvested and lysed. 40 µg of the cell lysate was separated in 12% polyacrylamide gel, transferred to a nitrocellulose membrane, and immunoblotted with antibodies against (A) caspase-7, -9, and -3 and substrate PARP; and (B) Bcl-2 family proteins, including Bcl-XL, Bcl-2, and Bax. Data are representative of three independent experiments.