Figure 9. Immunofluorescence microscopic images of treating DU145 cells with Andro and Taxi on the spindle-chromosome configuration.

The cells were treated with 20 µM of Andro in the presence and absence of 100 µM of Taxi for 24 h. The treated cells were fixed and immunostained with anti-tubulin antibody and FITC conjugated secondary antibody to visualize microtubules (green) and Hoechst 33342 to visualize chromosomes (blue). Representative images of spindle organization in the cells treated with vehicle control (hollow arrow), Andro alone (solid arrows) and combined Andro and Taxi (dashed arrows) are shown. Bar = 10 µm.