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. 2013 Jan 28;8(1):e54577. doi: 10.1371/journal.pone.0054577

Figure 11. The effect of MAD2 knockdown on the mitotic index of DU145 cells treated with Andro and/or Taxi.

Figure 11

Cells were either treated with Andro and/or Taxi for 24 h or transfected with the negative control siRNA or MAD2 specific siRNA for 24 h and later treated with Andro and/or Taxi for additional 24 h. Then cells were fixed and stained with anti-phospho-histone H3 (Ser10) antibody, FITC-conjugated secondary antibody and PI, and analyzed by flow cytometry. Mitotic index is expressed as mean ± s.d. * indicates significant difference between two groups, *p<0.01.