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. 2013 Jan 28;8(1):e54586. doi: 10.1371/journal.pone.0054586

Figure 7. HSV-1-encoded gB signals through MyD88/TRAF6, but not TRAF2.

Figure 7

HEK293T cells were transiently transfected with pCMV1-FLAG-hTLR2 and the NF-κB luciferase reporter plasmid along with vector (pCMV-HA or pCMV-FLAG) and various concentrations of pHA-DN-MyD88 (A), pDN-TRAF6 (B) or pFLAG-DN-TRAF2 (C). After 24–36 h, cells were stimulated with gBs (20 µg/ml) or zymosan (10 µg/ml) or left untreated for 8 h, and luciferase reporter gene activity was measured as described in Materials and Methods. Luciferase activity values are expressed as the ratio between firefly and Renilla luciferase. The results are shown as the mean ± SD of three independent experiments. Statistical analysis was performed using Student’s t test. (A) *, P = 0.011. (B) *, P = 0.023.