Table 1. Bacterial strains, plasmids and cell lines used in this study.

Enteropathogenic E. coli strain	Description	Source/Reference
Wild type EPEC	Prototypical strain E2348/69	[38]
Δmap	Lacking the map gene	[18]
ΔespF	Lacking the espF gene	[39]
Δquad	Lacking map, espF, tir and eae (encodes Intimin) genes	[40]
ΔmapespF	Lacking map and espF genes	[17]
ΔTTSS	Type three secretion mutant deletion for espA	[41]
Plasmids	Description	Source/Reference
pd2-CMV-EGFP	Mammalian EGFP expression	This study
pEGFP-MITO	Mitochondrial targeting sequence of cytochrome c fused to EGFP	[23]
pespF	pBR322 vector for bacterial expression of espF	[20]
p(L16E)espF	pBR322 vector for bacterial expression of espF missing the mitochondrial localisation sequence	[20]
pespF(D3)	the critical arginines of espF in the three SNX9 motifs exchanged for aspartic acid	[28]
pespF (A3)	espF expression with all three critical leucine in the N-WASP binding motif exchanged for alanine	This study
pespF (A2)	As above, with only 2 leucine exchanged – L104A and L197A	This study
pespF (A1)	As above, with only 1 leucine exchanged – L104A	This study
pTccp	Bacterial expression – espF homologue Tccp	[23]
pSK-map	pBluescript based bacterial expression vector for map	[18]
pSK-mapΔTRL	pSK-map with the C-terminal DTRL amino acids removed	This study
pSK-mapΔE78A	pSK-map with the E78A substitution	Thus study
Cell lines	Description	Source/Reference
Caco-2	Heterogeneous model for small intestinal enterocytes.	ATCC HTB-37
TC-7	Homogeneous Caco-2 clone	[10]
HeLa	Human cervical carcinoma cells	ATCC No. CCL-2
Clones NCL1-NCL12	Caco-2 clones	This study
BBE1	Caco-2 clone with dense brush border	[11]
Primers
P1-E78A	GCAATGGTTCAAGCAAGCGCAGATTACTTTTCTATCC
P-ΔTRL PS	CGGAATTCATGTTTAGTCCAACGGC
P-ΔTRL NS	CGGTCGACCTAATCCTGCACATTGTC
P-EspF NW NS	CGATAGTTCATAGGCAGCTGCTGCATGATCTTTTAGTGCCTGTGC