Figure 5. H₂O₂ induced oxidative stress stimulates increased ubiquitination of C. neoformans proteins.

(A): Immuno blot analysis of the proteins from C. neoformans cells growing in YNB, pH 4 at OD₆₅₀ = 1.5 and treated with various concentrations (0, 1, 2 and 4 mM) of H₂O₂. Cell lysates were prepared in urea containing denaturing buffer. Equal amount of protein was electrophoresed on SDS-polyacrylamide gel, transferred to nitrocellulose and ubiquitin conjugates were probed with polyclonal anti-ubiquitin antiserum. (B): Densitometric quantification of the signal from immunoblot A.