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. 2013 Jan 28;8(1):e55018. doi: 10.1371/journal.pone.0055018

Figure 2. Dengue virus induces MIF production in monocytes and human umbilical cord vein endothelial cells (HUVECs).

Figure 2

THP-1 cells or primary HUVECs were infected with DV2 (MOI = 10). (A, B) The concentrations of MIF in culture media were assayed at 48 h post-infection using ELISA kits as described in the Materials and Methods. Controls were uninfected cells (Mock) and cells treated with UV-inactivated DV (UVDV2). (C) DV2 induced MIF mRNA expression in HUVECs. HUVECs were infected with DV2 (MOI = 1 or 10) and incubated for 6 hours. RNA was extracted and the expression of MIF was analyzed by semi-quantitative RT-PCR with specific primers for MIF. The gene expression of GADPH was used as the internal control. Controls were cells without virus (Mock) and cells treated with UV-inactivated DV (UV).