Figure 6. rMIF enhances ICAM-1 and TM expression via the Erk, JNK MAPK and the PI3K signaling pathways in THP-1 cells.

(A) Erk inhibitor U0126, (B) JNK inhibitor SP60125, or (C) PI3K inhibitor LY294002 at 20 µM in DMSO was added to the THP-1 cell culture 30 min before and throughout rMIF treatment. The cells (2×10⁶) were stimulated with rMIF (0.4 µg/mL) for 24 h. The expression of ICAM-1 (Left panel) and TM (Right panel) was assessed by flow cytometry analysis. The rMIF-treated cells were compared to the controls.