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| Primer name | sequence |
| Luc-fwd | CCGAGCTCAGGAGATATCATGGAAGACGCCAAAAAC |
| Luc-rev | GGCCATGGTCACAATTTGGACTTTCCGCCC |
| EcoRI-fwd | GCACTGATAATGAACTCCTCTGGATCTACTGGG |
| EcoRI-rev | CCCAGTAGATCCAGAGGAGTTCATTATCAGTGC |
| SphI-fwd | GCGTCAGATTCTCGCACGCCAGAGATC |
| SphI- rev | GATCTCTGGCGTGCGAGAATCTGACGC |
| metK-SphI-fwd | GCATGC AGTTGATTTTAACTAACTTTTGCT |
| metK-SacI-rev | GAGCTC ATTTAAAATGAACCACAATTGTATC |
Restriction site are underlined. Nucleotides used to delete restriction sites are in bold.
